The man, who works for Norfolk-based company Konectbus, was said to be unhappy about the bus’s number being multicoloured in a nod to the LGBT rainbow flag.
Passenger Rebecca Sears said she was kept waiting as the driver insisted on swapping vehicles at Norwich Bus Station, two weeks after the city celebrated a Pride event.
The 19-year-old was trying to catch the 501 park and ride service to Thickthorn on August 9 when the alleged incident unfolded.
“He said we have to wait a minute, he said I am not driving this bus because it promotes homosexuality,” she said.
“I was standing with two other women and we thought he was joking.”
She added: “I just hope that he hasn’t said that in front of somebody that is gay. I would hate to think he would say something to them.
“He didn’t know if I was gay or the other two women I was with. He just out right said it.”
Miss Sears immediately complained about the driver’s behaviour to staff at the front desk.